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The NKI shRNA Library

shRNA library targeting 8000 human genes including:
  • Cell cycle genes
  • Oncogenes and tumorsuppressor genes
  • Transcription factors
  • Kinases and phosphatases
  • Signal transduction
  • Chromatin structure (43 genes)
  • DNA repair and replication (71 genes)
  • Adhesion
  • Angiogenesis
  • Apoptosis
  • Genes implicated hereditary cancer
  • Genes implicated drug response
  • Disease genes
  • etc...
Generated 3 different shRNA constructs against each gene:
  • 24.000 unique 19_mer sequences
  • 24.000 pRETROSUPER shRNA constructs
  • 48.000 59_mer oligos (~ 500 96 well plates oligos)
~ 80 96 well plates with bacterial glycerol stocks:
  • each well 1 gene (3 shRNA constructs)
  • each plate 96 genes (288 shRNA constructs)
  • 4.000 shRNA constructs ( 8000 wells)
DNA isolated from :
  • single well / single gene (8000)
  • per 96 well plate (~ 80 pools of 96 genes)
  • subsets of genes of interest (gene families)
  • total library







 

Updated 13/Feb/06